Our technology

A custom optical instrument sensitive enough to detect individual 20 nm particles

Common optical instruments are not sensitive enough to detect nanoparticles smaller than 60 nm because such small particles scatter so little light . We have built our instrument around a new interferometric microscopy technique that allows to increase the signal by 4 orders of magnitude, unveiling the smallest virus, EVs and LNP you want to work with

Measure an absolute concentration as well as the size and mass of each and every single of your nanoparticles

The definition of concentration is the number of particles per unit volume. Our microscope uses holographic imaging principles to not only record 2D images, but 3D images from a small volume within the sample. Our algorithm can explore this volume, count the number of particles present in it and deduce an absolute concentration measurement, without any assumptions, suppositions or risks associated with the use of indirect measurements.

Theory of light diffraction states that nanoparticles small enough (small compared to wavelength ie 450 nm) scatter an amount of light that is proportional to the square of their “polarizability “. This polarizability, for small particles, is proportional to their mass. In practice, this means that heavier particles scatter more light, and appear brighter on our images as illustrated in the video. We use this property by having our software measure the brightness of each and single nanoparticles on the screen and deduce their mass.  Those masses are used to build the 2 dimensions mass and hydrodynamic radius distribution. 

Every second, 1000 « 3D snapshots »  of the individual nanoparticles are generated by our instrument. Our software automatically tracks those particles and reconstruct their trajectory. The analysis of each trajectory enables to derive an hydrodynamic diameter for each particle. The output is used to build the 2 dimensions hydrodynamic diameter distribution.

Quantitative measurements
of vector loading or particle aggregation

Our instrument measures a quantitative concentration, as well as the mass and size distribution of nanoparticles in the sample. Because vector that are full, are heavier than empty vectors, they can be distinguished on the mass histogram. With one look on the mass histogram, you can distinguish the full and empty vectors as well as rely on our software to estimate the full/empty ratio

Measure all of your R&D conditions in minutes

Optics is one of the pillar of sensing technologies for biologics production because of the richness of the information is can provide simply without altering the sample. Our instruments is label free, requires only 5 µL of sample, and takes 5 minutes to produce a result. Simply take a drop of the condition you want to test, put it in the instrument, click play, and get you quantitative result without manual fine tuning of parameter